Science Trader

Honest Science, All the Time

 

DNA/RNA Technology and Protocols

Extraction and Isolation of RNA from cells using TRIZOL reagent.  Higher Yield than RNA extraction kits.  RT-PCR suitable.

Basic Template for setting up restriction digests.

How to successfully design primers, obtain starting material and clone genes from the genome of the host of your choice.

The math behind the art of vector/insert ligations.

Purification of DNA after digestion/treatment or simple way of DNA concentration.

DNA ligations

Cloning of genes from Cells/Tissues

DNA Restriction Enzyme Digests