Science Trader

Honest Science, All the Time

 

Assay Protocols—Westerns, ELISAs, cell assays etc.  For everything assayable

Buffers, reagents and protocol for standard sandwich ELISA.

Recipe for making your own ECL substrate and saving loads of money

Stripping blots for re-probing, using harsh conditions that will remove bound antibody.

Alternative method of detecting HRP coupled antibodies on western blots.  Blue bands appear on blot, No film required.

IP of proteins from Lysates generated from Mammalian Cells.

Non-radioactive killing assay that only requires a fluorescent plate reader.

For antibodies with weaker affinities to proteins or with weak signals to begin with.