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Science Trader |
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Honest Science, All the Time |
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Mammalian Cell-culture Protocols |
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Transduction using viral supernatants from pMIG vector and PlatE cells. This is not a high efficiency transduction. |
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Another Transduction protocol requiring more time than Transduction 1. |
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Type a brief description of this item. |
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Protocol for activation and culture of T and NK cells for determining levels of cytokine secretion (IL-2, IFNgamma etc.). |
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Transfection of PlatE cells using ExGen500 by fermentas. This produces viral supernatants suitable for transduction. |
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Item name 5 |
