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Science Trader |
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Honest Science, All the Time |
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Here you can find protocols for a wide variety of biological research techniques and some common buffers. If you have comments on these protocols or would like to add your own protocols or modifications, please visit the discussion boards to comment on that protocol. |
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Useful Protocols |
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Competent cells, DNA transfer, DNA preparation techniques, Bacterial protein expression systems, Phage Display etc. |
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Competent cells, DNA transfer, Growth and culture, Yeast-expression, Yeast-display etc. |
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Growth and maintenance of mammalian cell lines. DNA transfer, Protein Expression |
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Animal work protocols. Isolation and culture of tissues or cells. |
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Hybridoma growth, Antibody and protein growth/purification, labeling, modification., re-folding, Bacteria, Fly cells, mammalian cell proteins. |
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Western blotting, ELISA, Protein quantitation, Flow Cytometry protocols. Thermal denaturation, affinity measurements etc. |
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Protocols regarding purification or modification of DNA and RNA. Mini-preps, digests etc. |
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Recipes for commonly used buffers. PBS, Lysis, SDS-PAGE etc. |
